General Information

  • ID:  hor003117
  • Uniprot ID:  A0SIF1
  • Protein name:  RLRF peptide 2
  • Gene name:  sNPF
  • Organism:  Anopheles gambiae (African malaria mosquito)
  • Family:  NPY family
  • Source:  animal
  • Expression:  Expressed in all body parts of larva, pupae and adults.
  • Disease:  NA
  • Comments:  NA
  • Taxonomy:  gambiae species complex, Pyretophorus, Cellia (subgenus), Anopheles (genus), Anophelinae (subfamily), Culicidae (family), Culicoidea (superfamily), Culicomorpha (infraorder), Nematocera (suborder), Diptera (order), Endopterygota (cohort), Neoptera (infraclass), Pterygota (subclass), Dicondylia, Insecta (class), Hexapoda (subphylum), Pancrustacea, Mandibulata, Arthropoda (phylum), Panarthropoda, Ecdysozoa, Protostomia, Bilateria, Eumetazoa, Metazoa (kingdom), Opisthokonta, Eukaryota (superkingdom), cellular organisms
  • GO MF:  GO:0005184 neuropeptide hormone activity
  • GO BP:  GO:0007218 neuropeptide signaling pathway; GO:0032095 regulation of response to food; GO:0040014 regulation of multicellular organism growth
  • GO CC:  GO:0005576 extracellular region

Sequence Information

  • Sequence:  AIRAPQLRLRF
  • Length:  11
  • Propeptide:  MYRINLTTFTLLLVLAVGSLMSESLHPSDGAINDLYEYLLQREYAAPVSYADHQIKRKAVRSPSLRLRFGRRSDPSVPLRPEEDELIDQKAIRAPQLRLRFGRNDPLWTSFNENALLEENFEKRAPSQRLRWGRSNLFGNLVNQFQQDDVMQQKTIRAPQLRLRFGRTDPSWAMYNEHQLTTGQQAQPANEASEKRAPTQRLRWGRSDPALAKDSSEDKALDVEESENTNADDK
  • Signal peptide:  MYRINLTTFTLLLVLAVGSLMS
  • Modification:  T11 Phenylalanine amide
  • Glycosylation:  NA
  • Mutagenesis:  NA

Activity

  • Function:  Has a role in inhibiting host-seeking behavior during a reproductive cycle
  • Mechanism:  NA
  • Cross BBB:  NA
  • Target:  NA
  • Target Unid:  NA
  • IC50: sNPF receptor:4.9nM
  • EC50: NA
  • ED50: NA
  • kd: NA
  • Half life: NA

Structure

  • Disulfide bond:  NA
  • Structure ID:  AF-Q8LC53-F1(AlphaFold_DB_ID)

  • Structure: (PDB_ID-from https://www.rcsb.org/; AlphaFold_DB_ID-from https://alphafold.ebi.ac.uk/; hordbxxxxxx_AF2.pdb was predicted structure by AlphaFold2; hordbxxxxxx_ESM.pdb was predicted structure by ESMFold)
    •    AF-Q8LC53-F1.pdbhor003117_AF2.pdbhor003117_ESM.pdb

Physical Information

Mass: 151919 Formula: C61H105N21O13
Absent amino acids: CDEGHKMNSTVWY Common amino acids: R
pI: 12.8 Basic residues: 3
Polar residues: 0 Hydrophobic residues: 6
Hydrophobicity: -0.91 Boman Index: -2894
Half-Life: 4.4 hour Half-Life Yeast: >20 hour
Half-Life E.Coli: >10 hour Aliphatic Index 124.55
Instability Index: 7912.73 Extinction Coefficient cystines: 0
Absorbance 280nm: 0

Literature

  • PubMed ID:  17140700
  • Title:  Characterization and Expression of the Short Neuropeptide F Receptor in the African Malaria Mosquito, Anopheles Gambiae